Amplicon sequencing on Illumina MiSeq of Aegilops geniculata: accessi... - SRA

SRX6356517: Amplicon sequencing on Illumina MiSeq of Aegilops geniculata: accession PI 551118
1 ILLUMINA (Illumina MiSeq) run: 43,129 spots, 26M bases, 17.1Mb downloads

Design: PCR primers of 20-26 bp were designed in exons encompassing intronic regions of about 400 bp for 48 low-copy nuclear loci, with an annealing temperature arounf 60 C. PCRs of all nuclear loci were carried simultaneously on 48.48 IFC plates following Fluidigm protocol. Amplicons were then pooled and barcoded by sample for paired-end (2x300 bp) Illumina MiSeq.

Submitted by: Institute of Biology

Study: Amplicon sequencing of low-copy nuclear loci in Aegilops species

PRJNA550182 • SRP211843 • All experiments • All runs

show Abstracthide Abstract

The dataset was produced to infer the evolutionary history of four diploid Aegilops spp. (Ae. tauschii, Ae. umbellulata, Ae. caudata and Ae. comosa) and of four of their derivative allopolyploid species (Ae. cylindrica, Ae. crassa, Ae. geniculata and Ae. triuncialis). The dataset includes 420 accessions of these eight species, 29 replicates and 26 outgroup species: all other extent diploid Aegilops spp., Triticum monococcum, Triticum urartu, Secale cereale, Hordeum vulgare and Taeniatherum caput-medusae.

Sample: Sample names are read as follows: "IndividualName_AccessionID_AccessArrayBarcode"

SAMN12110126 • SRS5019389 • All experiments • All runs

Organism: Aegilops geniculata

Library:

Name: CGTAATGAGC

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 43,129 spots, 26M bases, 17.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR9590608	43,129	26M	17.1Mb	2020-07-22

ID:: 8412265

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Sequence Read Archive

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